UJI AKTIVITAS EKSTRAK LABU KUNING (Cucurbita maxima D) SEBAGAI KANDIDAT ANTI ACNE DAN ANTI FUNGI

Abstract

Introduction: Skin disorders that often appear and bother include tinea versicolor and acne. The presence of Pityriasis versicolor fungal infection or called tinea versicolor is caused by Malassezia furfur while acne occurs due to chronic inflammation that occurs in the pilosebaceous unit due to Propionibacterium acnes bacteria. The use of natural ingredients as an alternative treatment for fungal infections is increasing, one of which is using pumpkin fruit, which contains flavonoid compounds.Objective: This study aims to evaluate the activity of pumpkin (Cucurbita maxima D) fruit extract against Malassezia furfur and Propionibacterium acnes.Methods: This research is a pure experimental research design with a post test control group design. The paper disc diffusion method was used to evaluate the diameter of the inhibition zone of pumpkin extract (Cucurbita maxima D) with a concentration of 5% and 10% which was marked by the presence of a clear zone around the disc paper. Inhibition zone diameter data for both bacteria and fungi were measured, calculated and then compared to obtain anti-bacterial and anti-fungal activity.Results: Pumpkin extract with a concentration of 5% had very weak activity against the inhibition of Malassezia furfur and Propionibacterium acnes bacteria with an inhibition zone diameter of 2.73 ± 0.25 mm and 4.63 ± 5.34 mm. At a concentration of 10% began to have activity for the inhibition of the fungus Malassezia furfur with an inhibition zone diameter of 9.30 ± 1.22 mm and for inhibition of Propionibacterium acnes 11.83 ± 1.87 mm. In the pumpkin extract, there are metabolites of vitexin, azaleatin, isovitexin, quercetin, apigenin and rich in flavones. It has not been found comparable concentrations with ketoconazole and clindamycin in inhibition against Malassezia furfur and Propionibacterium acnes.Conclusion: Pumpkin extract has the potential to be anti-fungal and anti-acne but nothing is comparable to clindamycin so that it can be used as an anti-acne cosmetic formulation. The metabolite content of apigenin and quercetin in the extract has an effect on anti-fungal and anti-acne activity.